Abstract
Alcohol use disorder (AUD) is a chronic relapsing disorder characterized by an impaired ability to stop or control alcohol use despite adverse social, occupational, or health consequences. AUD is associated with a variety of physiological changes and is a substantial risk factor for numerous diseases. We aimed to characterize systemic alterations in immune responses using a well-established mouse model of chronic intermittent alcohol exposure to induce alcohol dependence. We exposed mice to chronic intermittent ethanol vapor for 4 weeks and analyzed the expression of cytokines IFN-γ, IL-4, IL-10, IL-12 and IL-17 by different immune cells in the blood, spleen and liver of alcohol dependent and non-dependent control mice through multiparametric flow cytometry. We found increases in IFN-γ and IL-17 expression in a cell type- and organ-specific manner. Often, B cells and neutrophils were primary contributors to increased IFN-γ and IL-17 levels while other cell types played a secondary role. We conclude that chronic alcohol exposure promotes systemic pro-inflammatory IFN-γ and IL-17 responses in mice. These responses are likely important in the development of alcohol-related diseases, but further characterization is necessary to understand the initiation and effects of systemic inflammatory responses to chronic alcohol exposure.
Funder
National Institute on Alcohol Abuse and Alcoholism
Pearson Center for Alcoholism and Addiction Research
Scripps Research Institute
Publisher
Public Library of Science (PLoS)
Reference63 articles.
1. Alcoholic disease: liver and beyond;A Rocco;World J Gastroenterol,2014
2. Alcohol and Cancer: Mechanisms and Therapies;A Ratna;Biomolecules,2017
3. Infection and Alcoholic Liver Disease;C Chan;Clin Liver Dis,2016
4. Increased Serum IgE in Alcoholics: Relationship With Th1/Th2 Cytokine Production by Stimulated Blood Mononuclear Cells;MJ Domínguez-Santalla;Alcoholism: Clinical and Experimental Research,2001
5. ERK1/2 and Egr-1 contribute to increased TNF-α production in rat Kupffer cells after chronic ethanol feeding;R Kishore;American Journal of Physiology-Gastrointestinal and Liver Physiology,2002
Cited by
16 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献