Characterisation of theFusarium graminearum-Wheat Floral Interaction

Author:

Brown Neil A.1,Bass Chris1,Baldwin Thomas K.1,Chen Huaigu1,Massot Fabien1,Carion Pierre W. C.1,Urban Martin1,van de Meene Allison M. L.1,Hammond-Kosack Kim E.1

Affiliation:

1. Department of Plant Pathology and Microbiology, Centre for Sustainable Pest and Disease Management, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK

Abstract

Fusarium Ear Blight is a destructive fungal disease of cereals including wheat and can contaminate the crop with various trichothecene mycotoxins. This investigation has produced a newβ-glucuronidase (GUS) reporter strain that facilitates the quick and easy assessment of plant infection. The constitutively expressedgpdA:GUSstrain ofFusarium graminearumwas used to quantify the overall colonisation pattern. Histochemical and biochemical approaches confirmed, in susceptible wheat ear infections, the presence of a substantial phase of symptomless fungal growth. Separate analyses demonstrated that there was a reduction in the quantity of physiologically active hyphae as the wheat ear infection proceeded. A simplified linear system of rachis infection was then utilised to evaluate the expression of severalTRIgenes by RT-qPCR. Fungal gene expression at the advancing front of symptomless infection was compared with the origin of infection in the rachis. This revealed thatTRIgene expression was maximal at the advancing front and supports the hypothesis that the mycotoxin deoxynivalenol plays a role in inhibiting plant defences in advance of the invading intercellular hyphae. This study has also demonstrated that there are transcriptional differences between the various phases of fungal infection and that these differences are maintained as the infection proceeds.

Funder

British Society for Plant Pathology

Publisher

Hindawi Limited

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