Genomic characterisation of respiratory syncytial virus: a novel system for whole genome sequencing and full-length G and F gene sequences-Reference-Cited by-同舟云学术

Genomic characterisation of respiratory syncytial virus: a novel system for whole genome sequencing and full-length G and F gene sequences

Published:2023-12-07 Issue:49 Volume:28 Page:
ISSN:1560-7917
Container-title:Eurosurveillance
language:en
Short-container-title:

Author:

Iglesias-Caballero María¹²^ORCID,Camarero-Serrano Sara²,Varona Sarai³,Mas Vicente²,Calvo Cristina⁴⁵^ORCID,García María Luz⁴⁶,García-Costa Juan⁷,Vázquez-Morón Sonia⁸²^ORCID,Monzón Sara³,Campoy Albert²,Cuesta Isabel³,Pozo Francisco⁸²^ORCID,Casas Inmaculada¹⁸²^ORCID

Affiliation:

1. These authors contributed equally

2. Laboratory of Reference and Research in Respiratory Viruses, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Spain

3. Bioinformatics Unit, Unidades Centrales Científico Técnicas, Instituto de Salud Carlos III, Majadahonda, Spain

4. CIBER de Enfermedades Infecciosas (CIBERINFEC), ISCIII, Madrid, Spain

5. Paediatric Infectious and Tropical Diseases Department, Hospital Universitario La Paz, Hospital La Paz Institute for Health Research (IdiPAZ Foundation), Madrid, Spain

6. Paediatric Department, Severo Ochoa University Hospital, Leganés, Biomedical Sciences Research Institute, Puerta de Hierro-Majadahonda University Hospital, Madrid, Spain

7. Santa María Nai Hospital, Ourense, Spain

8. CIBER de Epidemiología y Salud Pública (CIBERESP), ISCIII, Madrid, Spain

Abstract

To advance our understanding of respiratory syncytial virus (RSV) impact through genomic surveillance, we describe two PCR-based sequencing systems, (i) RSVAB-WGS for generic whole-genome sequencing and (ii) RSVAB-GF, which targets major viral antigens, G and F, and is used as a complement for challenging cases with low viral load. These methods monitor RSV genetic diversity to inform molecular epidemiology, vaccine effectiveness and treatment strategies, contributing also to the standardisation of surveillance in a new era of vaccines.

Publisher

European Centre for Disease Control and Prevention (ECDC)

Subject

Virology,Public Health, Environmental and Occupational Health,Epidemiology

Reference23 articles.

1. First RSV vaccine approvals.;Venkatesan;Lancet Microbe,2023

2. A highly potent extended half-life antibody as a potential RSV vaccine surrogate for all infants.;Zhu;Sci Transl Med,2017

3. Nirsevimab for prevention of RSV in healthy late-preterm and term infants.;Hammitt;N Engl J Med,2022

4. European Medicines Agency (EMA). Beyfortus. Amsterdam: EMA; 2022. Available from: https://www.ema.europa.eu/en/medicines/human/EPAR/beyfortus

5. RSVAB WGS and GF protocols. Majadahonda: Instituto de Salud Carlos III; 2023. dx.doi.org/10.17504/protocols.io.kqdg3xbzqg25/v1

Cited by 2 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. RSVAB WGS and GF protocols v2;2023-12-14

2. Respiratory syncytial virus infections – recent developments providing promising new tools for disease prevention;Eurosurveillance;2023-12-07