Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022-Reference-Cited by-同舟云学术

Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022

Published:2022-09-01 Issue:35 Volume:27 Page:
ISSN:1560-7917
Container-title:Eurosurveillance
language:en
Short-container-title:

Author:

Paran Nir¹,Yahalom-Ronen Yfat¹,Shifman Ohad²,Lazar Shirley²,Ben-Ami Ronen³⁴,Yakubovsky Michal⁴,Levy Itzchak³⁵,Wieder-Feinsod Anat³⁵,Amit Sharon⁶,Katzir Michal³⁷,Carmi-Oren Noga⁸,Levcovich Ariela⁸,Hershman-Sarafov Mirit⁹¹⁰,Paz Alona⁹¹⁰,Thomas Rebecca¹¹,Tamir Hadas¹,Cherry-Mimran Lilach¹,Erez Noam¹,Melamed Sharon¹,Barlev-Gross Moria¹,Karmi Shay¹,Politi Boaz¹,Achdout Hagit¹,Weiss Shay¹,Levy Haim¹,Schuster Ofir¹,Beth-Din Adi²,Israely Tomer¹

Affiliation:

1. Department of Infectious Diseases, Israel Institute for Biological Research, Ness-Ziona, Israel

2. Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona, Israel

3. Sackler medical school, Tel Aviv University, Tel Aviv, Israel

4. Infectious Diseases Department, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel

5. Infectious Disease Unit, Sheba Medical Center, Ramat Gan, Israel

6. Clinical Microbiology, Sheba Medical Center, Ramat Gan, Israel.

7. Infectious Disease unit, Meir Medical Center, Kfar Saba, Israel

8. Infectious Disease unit, Shamir (Assaf Harofeh) Medical Center, Be'er Ya'akov, Israel

9. Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel

10. Infectious Disease and Control Unit, Bnai Zion Medical Center, Haifa, Israel

11. Department of Emergency Medicine, Rabin Medical Center, Beilinson Hospital, Petah Tikva, Israel

Abstract

The current monkeypox virus global spread and lack of data regarding clinical specimens’ infectivity call for examining virus infectivity, and whether this correlates with results from PCR, the available diagnostic tool. We show strong correlation between viral DNA amount in clinical specimens and virus infectivity toward BSC-1 cell line. Moreover, we define a PCR threshold value (Cq ≥ 35, ≤ 4,300 DNA copies/mL), corresponding to negative viral cultures, which may assist risk-assessment and decision-making regarding protective-measures and guidelines for patients with monkeypox.

Publisher

European Centre for Disease Control and Prevention (ECDC)

Subject

Virology,Public Health, Environmental and Occupational Health,Epidemiology

Reference13 articles.

1. Centers for Disease Control and Prevention (CDC). 2022 Outbreak Cases & Data. Atlanta: CDC; 22 Aug 2022. Available from: https://www.cdc.gov/poxvirus/monkeypox/response/2022

2. Monkeypox: a global wake-up call.;Lancet,2022

3. Epidemiological, clinical and virological characteristics of four cases of monkeypox support transmission through sexual contact, Italy, May 2022.;Antinori;Euro Surveill,2022

4. Monkeypox: A New Threat?;Kmiec;Int J Mol Sci,2022

5. Human monkeypox.;McCollum;Clin Infect Dis,2014

Cited by 53 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Role of Direct Sexual Contact in Human Transmission of Monkeypox Virus, Italy;Emerging Infectious Diseases;2024-09

2. Addressing poxvirus challenges in the Middle East to enhance biosafety and biosecurity measures;Journal of Biosafety and Biosecurity;2024-09

3. Clinical and laboratory predictors of mpox severity and duration: an Italian multicentre cohort study (mpox-Icona);eBioMedicine;2024-09

4. Clinical presentation, viral shedding, and neutralizing antibody responses of mpox cases in South Korea: Single center experience;Journal of Clinical Virology;2024-08

5. A Label-free Optical Biosensor-Based Point-of-Care Test for the Rapid Detection of Monkeypox Virus;2024-07-05