Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024-Reference-Cited by-同舟云学术

Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024

Published:2024-08-08 Issue:32 Volume:29 Page:
ISSN:1560-7917
Container-title:Eurosurveillance
language:en
Short-container-title:

Author:

Schuele Leonard¹²^ORCID,Masirika Leandre Murhula¹³⁴⁵,Udahemuka Jean Claude¹⁶⁷,Siangoli Freddy Belesi⁸,Mbiribindi Justin Bengehya⁸,Ndishimye Pacifique⁹⁶,Aarestrup Frank M¹⁰,Koopmans Marion²,Oude Munnink Bas B²,Molenkamp Richard²,

Affiliation:

1. These authors contributed equally to this work and share first authorship

2. Department of Viroscience, Erasmus University Medical Center, Rotterdam, the Netherlands

3. Congo Outbreaks, Research for Development, South Kivu, Bukavu, Democratic Republic of the Congo

4. SaBio Instituto de Investigación en Recursos Cinegéticos IREC (Universidad de Castilla-La Mancha & CSIC), Ciudad Real, Spain

5. Centre de Recherche en Sciences Naturelles de Lwiro, South Kivu, DS Bukavu, Democratic Republic of the Congo

6. Genomics Research and Development Division, Stansile Research Organization, Kigali, Rwanda

7. Department of Veterinary Medicine, University of Rwanda, Nyagatare, Rwanda

8. Division Provinciale de la Santé, South Kivu, Bukavu, Democratic Republic of the Congo

9. Research and Innovation Centre, African Institute for Mathematical Sciences (AIMS), Kigali, Rwanda

10. Research Group for Genomic Epidemiology, National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark

Abstract

Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.

Publisher

European Centre for Disease Control and Prevention (ECDC)

Reference15 articles.

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1. Border control strategies for reducing importation risk of Clade Ib Mpox;2024-09-10

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3. Note from the editors: WHO declares mpox outbreak a public health emergency of international concern;Eurosurveillance;2024-08-15