Genomic characterisation of respiratory syncytial virus: a novel system for whole genome sequencing and full-length G and F gene sequences-Reference-Cited by-同舟云学术

Genomic characterisation of respiratory syncytial virus: a novel system for whole genome sequencing and full-length G and F gene sequences

Published:2023-12-07 Issue:49 Volume:28 Page:
ISSN:1560-7917
Container-title:Eurosurveillance
language:en
Short-container-title:

Author:

Iglesias-Caballero María¹²^ORCID,Camarero-Serrano Sara²,Varona Sarai³,Mas Vicente²,Calvo Cristina⁴⁵^ORCID,García María Luz⁴⁶,García-Costa Juan⁷,Vázquez-Morón Sonia⁸²^ORCID,Monzón Sara³,Campoy Albert²,Cuesta Isabel³,Pozo Francisco⁸²^ORCID,Casas Inmaculada¹⁸²^ORCID

Affiliation:

1. These authors contributed equally

2. Laboratory of Reference and Research in Respiratory Viruses, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Spain

3. Bioinformatics Unit, Unidades Centrales Científico Técnicas, Instituto de Salud Carlos III, Majadahonda, Spain

4. CIBER de Enfermedades Infecciosas (CIBERINFEC), ISCIII, Madrid, Spain

5. Paediatric Infectious and Tropical Diseases Department, Hospital Universitario La Paz, Hospital La Paz Institute for Health Research (IdiPAZ Foundation), Madrid, Spain

6. Paediatric Department, Severo Ochoa University Hospital, Leganés, Biomedical Sciences Research Institute, Puerta de Hierro-Majadahonda University Hospital, Madrid, Spain

7. Santa María Nai Hospital, Ourense, Spain

8. CIBER de Epidemiología y Salud Pública (CIBERESP), ISCIII, Madrid, Spain

Abstract

To advance our understanding of respiratory syncytial virus (RSV) impact through genomic surveillance, we describe two PCR-based sequencing systems, (i) RSVAB-WGS for generic whole-genome sequencing and (ii) RSVAB-GF, which targets major viral antigens, G and F, and is used as a complement for challenging cases with low viral load. These methods monitor RSV genetic diversity to inform molecular epidemiology, vaccine effectiveness and treatment strategies, contributing also to the standardisation of surveillance in a new era of vaccines.

Publisher

European Centre for Disease Control and Prevention (ECDC)

Subject

Virology,Public Health, Environmental and Occupational Health,Epidemiology

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5. RSVAB WGS and GF protocols. Majadahonda: Instituto de Salud Carlos III; 2023. dx.doi.org/10.17504/protocols.io.kqdg3xbzqg25/v1

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