Misidentification of meticillin-resistant Staphylococcus aureus by the Cepheid Xpert MRSA NxG assay, the Netherlands, February to March 2021

Author:

Sabat Artur J1,Bathoorn Erik1,Chlebowicz-Fliss Monika A1,Akkerboom Viktoria1,Kamphuis Inge2,dos Santos Claudy Oliveira2,Friedrich Alexander W1

Affiliation:

1. University of Groningen, University Medical Center Groningen, Department of Medical Microbiology, Groningen, the Netherlands

2. Isala Hospital, Laboratory for Medical Microbiology and Infectious Diseases, Zwolle, the Netherlands

Abstract

We describe two false-negative results in the detection of meticillin-resistant Staphylococcus aureus (MRSA) of sequence type 398 and spa type t011 using the Cepheid Xpert MRSA NxG assay. The isolates were recovered in late February and early March 2021 from two patients in different hospitals in the northern Netherlands. Variations between the two isolate genomes indicate that this MRSA strain might have been spreading for some time and could have disseminated to other regions of the Netherlands and other European countries.

Publisher

European Centre for Disease Control and Prevention (ECDC)

Subject

Virology,Public Health, Environmental and Occupational Health,Epidemiology

Reference11 articles.

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3. PFGE diversity within the methicillin-resistant Staphylococcus aureus clonal lineage ST398.;Bosch;BMC Microbiol,2010

4. Novel erm(T)-carrying multiresistance plasmids from porcine and human isolates of methicillin-resistant Staphylococcus aureus ST398 that also harbor cadmium and copper resistance determinants.;Gómez-Sanz;Antimicrob Agents Chemother,2013

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