Affiliation:
1. Department of Chemical Pathology, Institute of Child Health, 30 Guilford Street, London WC1 1EH, U.K.
Abstract
1. The effect of MgCl2 concentration on the interaction of Alcian Blue 8GX and glycosaminoglycans in the urine of patients with mucopolysaccharidosis was studied by using a new quantitative micro method for the measurement of Alcian Blue–glycosaminoglycan complexes. This provided a means of measuring the critical electrolyte concentrations of urinary glycosaminoglycans. 2. Theoretical considerations based on the preceding paper (Whiteman, 1973) and experimental evidence provided here show that Alcian Blue 8GX may be used for the direct quantitative determination of total urinary glycosaminoglycans. The method is simple, requires sample volumes of 50μl or less, and gives results comparable with those obtained by other more complicated methods.
Subject
Cell Biology,Molecular Biology,Biochemistry
Cited by
150 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献