Glycoengineering Chinese hamster ovary cells: a short history-Reference-Cited by-同舟云学术

Glycoengineering Chinese hamster ovary cells: a short history

Published:2021-03-11 Issue:2 Volume:49 Page:915-931
ISSN:0300-5127
Container-title:Biochemical Society Transactions
language:en
Short-container-title:

Author:

Donini Roberto¹²,Haslam Stuart M.¹,Kontoravdi Cleo²^ORCID

Affiliation:

1. Department of Life Sciences, Imperial College London, London SW7 2AZ, U.K.

2. Department of Chemical Engineering, Imperial College London, London SW7 2AZ, U.K.

Abstract

Biotherapeutic glycoproteins have revolutionised the field of pharmaceuticals, with new discoveries and continuous improvements underpinning the rapid growth of this industry. N-glycosylation is a critical quality attribute of biotherapeutic glycoproteins that influences the efficacy, half-life and immunogenicity of these drugs. This review will focus on the advances and future directions of remodelling N-glycosylation in Chinese hamster ovary (CHO) cells, which are the workhorse of recombinant biotherapeutic production, with particular emphasis on antibody products, using strategies such as cell line and protein backbone engineering.

Publisher

Portland Press Ltd.

Subject

Biochemistry

Link

https://portlandpress.com/biochemsoctrans/article-pdf/49/2/915/909966/bst-2020-0840c.pdf

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