l-Lactate transport into rat heart mitochondria and reconstruction of the l-lactate/pyruvate shuttle

Abstract

In vitro reconstruction of the l-lactate/pyruvate shuttle has been performed, which allows NADH oxidation outside rat heart mitochondria. Such a shuttle occurs due to the combined action of the novel mitochondrial l-lactate/pyruvate antiporter, which differs from the monocarboxylate carrier, and the mitochondrial l-lactate dehydrogenase. The rate of l-lactate/pyruvate antiport proved to regulate the shuttle in vitro.