Sequence of residues 400–403 of bovine serum albumin

Abstract

A large tryptic peptide of bovine serum albumin (residues 377–582) was subjected to 32 cycles of Edman degradation to determine the sequence of the last remaining unknown segment of this protein. Residues 400–403 were identified as gly-Phe-Gln-Asn. Amide assignments were also made at positions 388 (glutamine), 389 (asparagine), 391 (aspartic acid) and 392 (glutamine).