Quantification of a potential anticonvulsant drug GIZh-298 in rat plasma by liquid chromatography-mass spectrometry-Reference-Cited by-同舟云学术

Quantification of a potential anticonvulsant drug GIZh-298 in rat plasma by liquid chromatography-mass spectrometry

Published:2022-12-21 Issue:3 Volume: Page:37-45
ISSN:2686-8830
Container-title:Pharmacokinetics and Pharmacodynamics
language:
Short-container-title:Fk&Fd

Author:

Bochkov P. O.¹^ORCID,Kravtsova O. Yu.¹^ORCID,Kolyvanov G. B.¹^ORCID,Litvin A. A.¹^ORCID,Boyko S. S.¹^ORCID,Zherdev V. P.¹^ORCID

Affiliation:

1. FSBI «Zakusov Insti¬tute of Pharmacology»

Abstract

GIZh-298 is a new derivative of 4-phenylpyrrolidone with anticonvulsant and nootropic effects. A selective and sensitive HPLC-MS technique for the quantitative determination of GIZh-298 in rat blood plasma has been developed and validated. The linearity of the technique was confirmed by a high correlation coefficient (>0.99). Recovery of GIZh-298 from blood plasma averaged 73.4 ±3.4 %. Accuracy during one working cycle and between cycles was < 4.01%, precision < 8.03 %. The study of the stability of GIZh-298 revealed that the target compound is stable in a biomatrix at room temperature (4 h), when it is in a thermostatically controlled autosampler (8 °C) during an analytical experiment, with prolonged storage at -50 ° C for 30 days, and also if it is subjected to several freeze-thaw cycles (3 cycle). The pharmacokinetics of GIZh-298 in rat blood plasma after a single intravenous injection at a dose of 60 mg/kg was studied.

Publisher

Publishing House OKI

Subject

Pharmacology (medical),Complementary and alternative medicine,Pharmaceutical Science

Reference8 articles.

1. Durnev AD, Voronina TA, Litvinova SA, Nerobkova LN, Gajdukov IO, Gudasheva TA, Zhmurenko LA, Mokrov GV. Pat. RF № 2643091 31.01.2018 4-benzoylpyridine oxime derivatives having anticonvulsant activity as agents for treatment of epilepsy and paroxysmal states (In Russ).]. URL: https://patenton.ru/patent/RU2643091C2. Ссылка активна на 15.09.2022

2. Zhmurenko LA, Mokrov GV, Nerobkova LN, Litvinova SA, Gaydukov IO, Voronina TA, Gudasheva TA. Novel 4-benzoylpyridine oxime derivative GIZH-298 with ati-convuisant activity. Farmakokinetika i farmakodinamika = Pharmacokinetics and pharmacodynamics. 2017;(1):22—26. (In Russ).].

3. Gaydukov IO, Litvinova SA, Voronina TA, Nerobkova LN, Zhmurenko LA, Mokrov GV, Avakyan GG, Kutepova IS. 4-Benzoylpyridine oxime derivative (GIZH-298) versus valproic acid: the anticonvulsant potential effect in a model of epilepsy in rats with cobalt-induced lesions. Epilepsy and paroxysmal conditions. [Epilepsiya i paroksizmal’nye sostoyaniya]. 2017;9(2)57—66. (In Russ).]. DOI: 10.17749/2077-8333.2017.9.2.057-066

4. Izuchenie bioekvivalentnosti vosproizvedennykh lekarstvennykh sredstv. Rukovodstvo po ekspertize lekarstvennykh sredstv. Tom I. - M.: Grifi K; 2013. (In Russ).].

5. Rukovodstva po ekspertize lekarstvennykh sredstv Tom I, pravil provedeniya issledovanii bioekvivalentnosti lekarstvennykh preparatov v ramkakh Evraziiskogo ekonomicheskogo soyuza, Sovet Evraziiskoi ekonomicheskoi komissi, reshenie ot 3 noyabrya 2016 No. 85. (In Russ).].

Cited by 1 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Pharmacokinetics of a potential antiepileptic drug GIZh-298;Pharmacokinetics and Pharmacodynamics;2024-08-08