Author:
Milanez Thais V.,Schoenlein-Crusius Iracema H.,Okino Luci K.
Abstract
Screening tests for aflatoxins B1, B2, G1 and G2, ochratoxin A and sterigmatocystin productionwere performed in 13 strains of Aspergillus spp, isolated from the terrestrial environment in the BrazilianAtlantic Rainforest (São Paulo State/Brazil). Coconut agar medium and moistened corn were employed assubstrates. The fungal extracts obtained from both media were submitted to thin-layer chromatography and the toxins were estimated according to the intensity of their fluorescence observed under UV light. None of the tested strains presented any of the mentioned mycotoxins. Because many unknown fluorescent spots were present, it was necessary to proceed a confirmation step using multiple chromatography, two dimensional chromatography and derivatization. In view of the accuracy of the employed methods and thepresence of many unknown fluorescent spots, the need of further studies on the production of others mycotoxins of fungi isolated under tropical conditions is justified.
Reference25 articles.
1. 1. Abarca, M.L. et al. New ochratoxigenic species in the genus Aspergillus. J. Food Prot., 60 (12): 1580-1582, 1997.
2. 2. Abarca, M.L. et al. Ochratoxin A production by strains of Aspergillus niger var. niger. Appl. Environ. Microbiol., 60: 2650-2652,1994.
3. 3. Arseculeratne, S.N. et al. Coconut as medium for the experimental production of aflatoxin. Appl. Microbiol., 18(1): 88-94, 1969.
4. 4. Bastos, S.T.G. Método rápido para detecção de produção de ocratoxina A e esterigmatocistina por Aspergillus spp em meios de ágar. Brasília, 1979, 77p. [Master’s dissertation - Instituto de Ciencias Biológicas, Universidade de Brasília].
5. 5. Ciegler, A. Bioprodution of ochratoxin A and penicillic acid by members of the Aspergillus ochraceus group. Can. J. Microbiol., 18: 631-636, 1972.