The dynamics of gonadotrophin-releasing hormone, LH and FSH secretion during the spontaneous ovulatory surge of the mare as revealed by intensive sampling of pituitary venous blood

Abstract

Abstract Conflicting views exist on the mode of gonadotrophinreleasing hormone (GnRH) secretion during the ovulatory LH surge and the relative importance of changes in pituitary responsiveness to GnRH in generating the LH surge. This disagreement may stem from species differences and/or methodological problems. To provide data on the exact relationship between GnRH and gonadotrophin secretion during the spontaneous LH surge, we collected pituitary venous (PV) blood every 30 s for 3–4 h from eight mares and then assayed GnRH (in six of the mares), FSH and LH. Jugular blood was also collected from twelve mares without PV cannulae either thrice daily during the surge (n =8) or hourly for 24 h when close to ovulation (n=4) and assayed for LH. Hormone peaks in PV blood were detected by the Cluster program and PV hormone patterns were scanned for underlying periodicity using spectral analysis. Jugular LH concentrations rose slowly and steadily without abrupt increase during the prolonged ovulatory surge, suggesting that hormone secretory patterns seen during the periods of rapid sampling were typical of the surge. Jugular LH concentrations were similar in mares with and without PV cannulae. Intensive sampling of PV blood showed that GnRH, FSH and LH were secreted in frequent (two to five per h) brief (5–7 min) peaks. Secretion was not detectable in 24%, 28% and 57% of the total sampling time for GnRH, LH and FSH respectively. GnRH and LH peaks appeared to be irregular in time and amplitude in most mares. However, spectral analysis of the data revealed an underlying periodicity in the secretion of all three hormones, with the dominant period ranging from 20 to 65 min in individual mares. The spectra of GnRH, FSH and LH were highly coherent at this dominant frequency, and 90% of GnRH peaks were concurrent with LH peaks, which is consistent with the dogma that GnRH is the primary secretagogue for both FSH and LH. Although PV FSH and LH concentrations were closely correlated, PV GnRH and gonadotrophin concentrations were only weakly correlated, implying that there was no consistent relationship between the magnitudes of changes in GnRH and gonadotrophin secretion. When compared with our published mid-luteal phase values, the daily GnRH secretion rate during the LH surge was trebled, while the LH responsiveness to endogenous GnRH, as assessed by the between newly secreted LH and PV GnRH concentrations, was four times greater. We conclude that during the mare's ovulatory LH surge (1) GnRH, LH and FSH are secreted together, probably discontinuously, in frequent brief peaks, with the same underlying periodicity, which implies the existence of a pulse generator, (2) changes in pituitary responsiveness play an important role in generating the mare's LH surge, and (3) GnRH is the major signal for both FSH and LH secretion; however, other factors acutely modulate pituitary response to GnRH. Journal of Endocrinology (1994) 140, 283–295