The use of surfagon for the induction of ovulation of mares in ART cycles

Abstract

In order to predict the timing of ovulation in mares, to obtain mature oocytes and follicular fluid in ART cycles, the domestic analogue of GnRH surfagon was used in two doses (40 and 50 mcg) against the background of comparison with the effectiveness of the previously used hCG drug. Surfagon injections were carried out intramuscularly when the leading follicle reached a diameter of 35 mm or more, and there was maximum or decreased edema of the uterus. An analysis of the duration of the intervals between intramuscular injection of 40 µg and 50 µg of the drug and ovulation showed that 72.4 and 35% of ovulations occur, respectively, between 37 and 48 hours after injection. The result of using 40 µg of surfagon is similar to the results of using hCG - 72 and 74% of ovulations in this period, respectively. Therefore, the drug “Surfagon” at a dose of 40 µg is most suitable for use in ART cycles. Only 3.4% of ovulations after the use of 40 µg of surfagon occur in the interval up to 24 hours after injection. Therefore, its use allows planning the collection of follicular fluid and oocyte from the preovulatory follicle using the OPU method after 24 hours. The domestic drug Surfagon (lyuleberin acetate, CJSC Mosagrogen, Moscow) is effective for the induction of ovulation in mares and can serve as an alternative to the use of HCG and imported GnRH analogues both in normal reproduction practice and in planning OPU procedures for the purpose of follicular fluid intake and oocyte extraction.