Novel method of hydrolysed collagen extraction from hide trimming waste

Abstract

Hide trimming waste is a by-product of tannery. Collagen is the main structural protein in hide trimming waste (70%), and extensively utilised in numerous industries including food, non-food, cosmetics, and medical. Research related to the development of hydrolysed collagen (h-collagen) extraction methods from cowhide, especially from the hide trimming waste, is still limited. The present work thus aimed to develop a four-step method for extracting h-collagen from hide trimming waste, and examine the product's properties. The present work successfully developed a method for extracting h-collagen from trimming waste with a 20.35% yield. Analysis of molecular weight, FTIR, and amino acid composition confirmed that the product was h-collagen with a molecular weight of 16 - 23 kDa. This h-collagen had higher antioxidant activity than commercial h-collagen, with an IC50 value of 238.5 ppm.