Systematic analysis of YFP traps reveals common mRNA/protein discordance in neural tissues

Author:

Titlow Joshua S.1ORCID,Kiourlappou Maria1ORCID,Palanca Ana1ORCID,Lee Jeffrey Y.1ORCID,Gala Dalia S.1ORCID,Ennis Darragh1ORCID,Yu Joyce J.S.1ORCID,Young Florence L.1ORCID,Susano Pinto David Miguel1ORCID,Garforth Sam1ORCID,Francis Helena S.1ORCID,Strivens Finn1ORCID,Mulvey Hugh1ORCID,Dallman-Porter Alex1ORCID,Thornton Staci1ORCID,Arman Diana1ORCID,Millard Marissa J.1ORCID,Järvelin Aino I.1ORCID,Thompson Mary Kay1ORCID,Sargent Martin2ORCID,Kounatidis Ilias1ORCID,Parton Richard M.1ORCID,Taylor Stephen2ORCID,Davis Ilan1ORCID

Affiliation:

1. University of Oxford 1 Department of Biochemistry, , Oxford, UK

2. Weatherall Institute for Molecular Medicine, University of Oxford 2 , Oxford, UK

Abstract

While post-transcriptional control is thought to be required at the periphery of neurons and glia, its extent is unclear. Here, we investigate systematically the spatial distribution and expression of mRNA at single molecule sensitivity and their corresponding proteins of 200 YFP trap lines across the intact Drosophila nervous system. 97.5% of the genes studied showed discordance between the distribution of mRNA and the proteins they encode in at least one region of the nervous system. These data suggest that post-transcriptional regulation is very common, helping to explain the complexity of the nervous system. We also discovered that 68.5% of these genes have transcripts present at the periphery of neurons, with 9.5% at the glial periphery. Peripheral transcripts include many potential new regulators of neurons, glia, and their interactions. Our approach is applicable to most genes and tissues and includes powerful novel data annotation and visualization tools for post-transcriptional regulation.

Funder

Wellcome Trust

Micron Oxford

Medical Research Council

Engineering and Physical Sciences Research Council

Biotechnology and Biological Sciences Research Council

Leverhulme Trust

A.G. Leventis Foundation

Zegami Ltd.

Publisher

Rockefeller University Press

Subject

Cell Biology

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