Chromosomes are predominantly located randomly with respect to each other in interphase human cells

Author:

Cornforth Michael N.1,Greulich-Bode Karin M.23,Loucas Bradford D.1,Arsuaga Javier45,Vázquez Mariel4,Sachs Rainer K.4,Brückner Martina3,Molls Michael3,Hahnfeldt Philip6,Hlatky Lynn6,Brenner David J.7

Affiliation:

1. Department of Radiation Oncology, University of Texas Medical Branch, Galveston, TX 77555

2. Department of Skin Carcinogenesis, German Cancer Research Center, Heidelberg 69120, Germany

3. Department of Radiation Oncology, Technical University of Munich, Munich 81675, Germany

4. Department of Mathematics, University of California, Berkeley, CA 94720

5. Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720

6. Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115

7. Center for Radiological Research, Columbia University, New York, NY 10032

Abstract

To test quantitatively whether there are systematic chromosome–chromosome associations within human interphase nuclei, interchanges between all possible heterologous pairs of chromosomes were measured with 24-color whole-chromosome painting (multiplex FISH), after damage to interphase lymphocytes by sparsely ionizing radiation in vitro. An excess of interchanges for a specific chromosome pair would indicate spatial proximity between the chromosomes comprising that pair. The experimental design was such that quite small deviations from randomness (extra pairwise interchanges within a group of chromosomes) would be detectable. The only statistically significant chromosome cluster was a group of five chromosomes previously observed to be preferentially located near the center of the nucleus. However, quantitatively, the overall deviation from randomness within the whole genome was small. Thus, whereas some chromosome–chromosome associations are clearly present, at the whole-chromosomal level, the predominant overall pattern appears to be spatially random.

Publisher

Rockefeller University Press

Subject

Cell Biology

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