Fatty acid compositions and antioxidant activities of Ranunculus isthmicus subsp. tenuifolius and Ranunculus rumelicus
-
Published:2022-11-18
Issue:
Volume:
Page:
-
ISSN:2602-2397
-
Container-title:International Journal of Nature and Life Sciences
-
language:en
-
Short-container-title:Int J Nature Life Sci
Author:
FAFAL Tuğçe1, SÜMER TÜZÜN Burcu2, KIVÇAK Bijen2
Affiliation:
1. EGE ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ 2. EGE ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ, ECZACILIK PR.
Abstract
The content of fatty acids in plants and especially the ratio of unsaturated to saturated fatty acids is very important. Phenolic compounds and flavonoids with antioxidant properties are useful in scavenging harmful radicals. In this study, fatty acid compositions and antioxidant activities of the aerial parts of Ranunculus isthmicus subsp. tenuifolius (syn. Xiphocoma tenuifolia) and Ranunculus rumelicus from Turkey were evaluated. Fatty acid methyl esters (FAMEs) of the oil extracts of two Ranunculus species were prepared. The fatty acid compositions of plants were investigated by gas chromatography (GC). Unsaturated fatty acids were detected in higher amounts than saturated fatty acids. The primary unsaturated fatty acids of the R. isthmicus subsp. tenuifolius oil were linoleic acid (38.42%), oleic acid (18.24%), palmitoleic acid (8.96%), and palmitic acid (8.12%) were determined fatty acid. Linoleic acid (24.09%), palmitoleic acid (16.25%) and palmitic acid (10.32%) were found in R. rumelicus oil as the major fatty acids. The antioxidant activities of methanol extracts of two plants were evaluated by DPPH•, ABTS•+, and CUPRAC assays. The extracts' total phenolics and flavonoid contents were determined by Folin-Ciocalteu and aluminium chloride methods, respectively. The methanolic extract of Ranunculus isthmicus subsp. tenuifolius demonstrated the higher antioxidant activity compared to R. rumelicus. The extracted oil from both Ranunculus species is a good source of essential fatty acids, especially linoleic acid. According to the antioxidant activity findings, it was determined that the extracts showed a significant correlation with the total phenolic and flavonoid contents.
Publisher
International Journal of Nature and Life Sciences
Subject
General Earth and Planetary Sciences,General Engineering,General Environmental Science
Reference31 articles.
1. Apak, R., Guclu, K., Ozyurek, M., & Karademir, S. E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry, 52(26), 7970-7981. https://doi.org/10.1021/jf048741x 2. Bhatti, M. Z., Ali, A., Ahmad, A., Saee, A., & Malik, S. A. (2015). Antioxidant and phytochemical analysis of Ranunculus arvensis L. extract. BMC Research Notes, 8, 279. https://doi.org/10.1186/s13104-015-1228-3 3. Campos, M. G., Webby, R. F., Markham, K. R., Mitchell, K. A., & da Cunha, A. P. (2003). Age-induced dimunition of free radical scavenging capacity in bee pollens and the contrubition of constituent flavonoids. Journal of Agricultural and Food Chemistry, 51(3), 742-745. https://doi.org/10.1021/jf0206466 4. Ceylan, R., Katanić, J., Zengin, G., Matić, S., Aktumsek, A., Boroja, T., Stanić, S., Mihailović, V., G. O., Boga, M., & Yılmaz, M. A. (2016). Chemical and biological fingerprints of two Fabaceae species (Cytisopsis dorycniifolia and Ebenus hirsuta): Are they novel sources of natural agents for pharmaceutical and food formulations? Industiral Crops and Products, 84, 254-262. https://doi.org/10.21448/ijsm.392354 5. Chang, C. C., Yang, M. H., Wen, H. M., & Chern, J. C. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10, 178-182.
|
|