Abstract
Milk Thistle Seeds (MTS) purifies the liver from all toxic and harmful substances, supports the regeneration of liver cells. In this study, the chemical composition of milk thistle seeds (Silybum marianum (L.) Gaertner = Carduus marianus L.) and also its biological activity were determined. Essential oil was analyzed using by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) techniques. After that, the essential oil was tested against bacteria and fungi by agar well diffusion and micro dilution methods. The essential oil yield was 1.1% (v/w). Eight constituents were comprised the 97.3% of the total oil extract of the Milk thistle seeds. The major compounds were determined as oleic acid (45.6%), linoleic acid (29.0%), ethylbenzene (7.0%) and stearic acid (5.7%). The seed essential oils of MTS significantly inhibited the growth of Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Escherichia coli and Streptococcus sp.) bacteria (p < 0.05). The oils also showed fungicidal activity against Candida tropicalis and C. globrata.
Publisher
International Journal of Nature and Life Sciences
Subject
General Earth and Planetary Sciences,General Engineering,General Environmental Science
Reference30 articles.
1. Adams, R. P. (2001). Identification of essential oil components by Gaschromatography/ Quadrupole Mass Spectroscopy. Carol Stream IL: Allured Publ Crop.
2. Ahmad, M., Zafar, M., Sultana, S., Azam, A., & Khan, A. M. (2014). The optimization of biodiesel production from a novel source of wild non-edible oil yielding plant Silybum marianum. International Journal of Green Energy, 11, 589-594.
3. Ahmad, N., Fazal, H., Abbasi , B. H., Anwar, S., & Basir, A. (2013). DPPH free radical scavenging activity and phenotypic difference in hepatoprotective plant (Silybum marianum). Toxicology and Industrial Health, 29, 460-467.
4. Alemardan, A., Karkanis, A., & Salehi, R. (2013). Breeding objectives and selection criteria for milk thistle [Silybum marianum (L.) Gaertn.]. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41, 340-347.
5. Andrzejewska, J., Sadowska, K., & Mielcarek, S. (2011). Effect of sowing date and rate on the yield and flavonolignan content of the fruits of milk thistle (Silybum marianum L. Gaertn.). Industrial Crops and Products, 33, 462-468.