Abstract
Protoplasts are microbial or vegetable cells lacking a cell wall. These can be obtained from microalgae by an enzymatic hydrolysis process in the presence of an osmotic stabilizer. In general, protoplasts are experimentally useful in physiological, geneticand bio-chemical studies, so their acquisition and fusion will continue to be an active research area in modern biotechnology. The fusion of protoplasts in microalgae constitutes a tool for strain improvement because it allows both intra and interspecific genetic recombina-tion, resulting in organisms with new or improved characteristics of industrial interest. In this review we briefly describe themethod-ology for obtaining protoplasts, as well as fusion methods and the main applications of microalgal platforms.
Publisher
Universidad Nacional de Colombia
Reference93 articles.
1. Abdel-Raouf, N., Al-Homaidan, A. A., & Ibraheem, I. B. M. (2012). Microalgae and wastewater treatment. Saudi Journal of Biological Sciences. https://doi.org/10.1016/j.sjbs.2012.04.005
2. Abomohra, A. E.-F., El-Sheekh, M., & Hanelt, D. (2016). Protoplast fusion and genetic recombination between Ochromonas danica (Chrysophyta) and Haematococcus pluvialis (Chlorophyta). Phycologia, 55(1), 65–71. https://doi.org/10.2216/15-88.1
3. Al-Zuhair, S., Ashraf, S., Hisaindee, S., Darmaki, N. Al, Battah, S., Svistunenko, D., … Chaudhary, A. (2017). Enzymatic pre-treatment of microalgae cells for enhanced extraction of proteins. Engineering in Life Sciences. https://doi.org/10.1002/elsc.201600127
4. Aoyagi, H. (2011). Application of plant protoplasts for the production of useful metabolites. Biochemical Engineering Journal, 56, 1–8. https://doi.org/10.1016/j.bej.2010.05.004
5. Araujo, K., Berradre, M., Rivera, J., Cáceras, A., Gisela, P., Aiello, C., & Pérez, D. (2016). Fusión intergénica de protoplastos de Saccharomyces cerevisiae y Hanseniaspora guillermondii. Revista de La Sociedad Venezolana de Microbiología, 51–57.
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