STUDY OF THE LIPID PIGMENT COMPOSITION IN THE WHITE SEA ALGAE SACCHARINA LATISSIMA USING TLC AND MALDI-MS

Author:

Krasnov К. А.1,Gladchuk A. S.2,Alexandrova M. L.1,Keltsieva O. A.3,Zaytseva M. A.1,Melnikova M. V.1,Reinyuk V. L.1,Podolskaya Е. P.4

Affiliation:

1. Institute of Toxicology of the Federal Medical Biological Agency

2. Institute of Toxicology of the Federal Medical Biological Agency; St. Petersburg State University

3. Institute of Analytical Instrumentation, Russian Academy of Sciences; Institute of Toxicology of the Federal Medical Biological Agency

4. Institute of Toxicology of the Federal Medical Biological Agency; Institute of Analytical Instrumentation, Russian Academy of Sciences

Abstract

The qualitative composition of the most important biologically active lipid substances-carotenoids and chlorophyll derivatives of the White sea algaeSaccharina latissimahas been studied. The lipid extract was separated by thin-layer chromatography (TLC) and examined by matrix-associated laser desorption-ionization with mass-spectrometric analysis (MALDI-MS). The extract contained fucoxanthin, fucoxanthinol, pheophytin a, pheophorbide a, as well as other carotenoids and chlorophylls, including those not described in the literature. The results obtained, which significantly expand the information about the composition of S. latissima pigments, can be used to standardize raw materials and preparations based on these algae.

Publisher

Russian Register of Potentially Hazardous Chemical and Biological Substances

Reference17 articles.

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