Dairy product enriched with triple manganese complex

Abstract

Micronutrient deficiency is a serious problem in Russia. In this article, we developed a complex of the essential microelement  manganese  with  ascorbic  acid  and L-isoleucine.  The  properties  of  the  resulting  compound were  studied  by  X-ray  phase  analysis,  IR  spectroscopy,  and  computer  quantum  chemical  modeling.  It  was established that the complex has an amorphous structure. As a result of the simulation, the optimal interaction model  was  determined:  through  the  carboxyl  group  and α-amino  group  of  L-isoleucine,  and  through  the  C2 and C3 atoms of ascorbic acid ( E = -2264.757 kcal/mol). Quantum chemical modeling data were confirmed by  IR  spectroscopy.  The  stability  of  the  complex  was  studied  under  various  technological  parameters  (pH, temperature  and  exposure  time).  To  do  this,  a  multifactorial  experiment  was  carried  out  with  three  input parameters  and  three  levels  of  variation.  It  has  been  shown  that  the  stability  of  the  ternary  complex  of  the essential microelement manganese with ascorbic acid and the essential amino acid L-isoleucine is significantly influenced  by  all  parameters  (pH,  temperature  and  exposure  time).  It  was  found  that  with  increasing  pH, temperature  and  exposure  time,  ∆  Eh  increases,  which  indicates  a  loss  of  stability  of  the  complex  and  its destruction. The parameters at which the stability of the samples is observed correspond to the lowest values of ∆  Eh: pH = 3–7,  t = 25–60°,  τ = 5–15. At the next stage, milk was enriched with the resulting compound at the  rate  of  30%  of  the  daily  dose  of  manganese  consumption  and  studied  the  physicochemical  parameters of  milk  depending  on  the  concentration  of  the  developed  complex.  It  was  found  that  for  the  enrichment of   manganese ascorbate isoleucinate it is necessary to use concentrations of 0.005 mol/l or less, since the obtained indicators correspond to the quality standards established in the Russian Federation. According to the organoleptic assessment, the values of the indicators (smell and taste) of milk enriched with manganese ascorbate isoleucinate exceed the values of the indicators of the control sample (JSC MKS, Stavropol, Russia) and the sample enriched with the inorganic form of iron — manganese sulfate.