Abstract
In this work, unmodified silver nanoparticles were synthesized by a simple and cost-efficient method and then characterized by TEM imaging and UV-Vis. spectroscopy. Thereafter, their nanozymatic activity was investigated by catalyzing the oxidation of 3,3’,5,5’-tetramethyl-benzidine (TMB) as the standard peroxidase substrate. The results exhibited a specific activity as high as 5.4 µM min-1 for the as-prepared unmodified silver nanoparticles. Afterward, the effect of daylight and air oxygen on the peroxidase-like activity of these nanozymes was checked within 7 days. The results revealed that the activity of unmodified silver nanoparticles was approximately retained at about 75%, and 63% after 7 days exposing daylight and air oxygen, in order. The shelf-self of the as-prepared nanozymes was also investigated at 4 ℃ under dark conditions, revealed that these nanozymes saved about 96% of their initial activity after 10 days of storage at 4 ℃ under dark conditions.
KEYWORDS:Unmodified silver nanoparticles; Peroxidase-like nanozymes; Air oxygen; Daylight; Shelf-stability
Reference24 articles.
1. 1. Hormozi Jangi S. R.; Akhond M. (2020). High throughput green reduction of tris (p-nitrophenyl) amine at ambient temperature over homogenous AgNPs as H-transfer catalyst. Journal of Chemical Sciences, 132, 1-8.
2. Hormozi Jangi, S. R., & Dehghani, Z. (2023). Spectrophotometric quantification of hydrogen peroxide utilizing silver nanozyme. Chemical Research and Nanomaterials. https://crn.shiraz.iau.ir/article_701960.html?lang=en.
3. Hormozi Jangi, S. R., & Akhond, M. (2021). Ultrasensitive label-free enantioselective quantification of d-/l-leucine enantiomers with a novel detection mechanism using an ultra-small high-quantum yield N-doped CDs prepared by a novel highly fast solvent-free method. Sensors and Actuators B: Chemical, 339, 129901.
4. Salehzadeh, A., Sadat Shandiz, A., & Naeemi, A. S. (2018). Cytotoxicity effectiveness of biosynthesized silver nanoparticles on breast cancer T47D cell line, using macro algae Laurencia caspica extract. Journal of Ilam University of Medical Sciences, 26(1), 52-61.
5. Hormozi Jangi, S. R. (2023). Synthesis and characterization of magnesium-based metal-organic frameworks and investigating the effect of coordination solvent on their biocompatibility. Chemical Research and Nanomaterials, 1(4), 1-9.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献