Abstract
The kinetics studies of the as-prepared BSA-gold nanozymes were performed by measuring their activity as a function of DAB or TMB (i.e., enzyme-substrate) concentrations. The kinetic parameter, Vmax and Km was then calculated by using Michaelis–Menten and the linear plot of Lineweaver–Burk for both substrates. the Vmax of DAB oxidation was found to be lower than that of the TMB oxidation which pointed to the fact that the catalytic efficiency of the as-prepared BSA-gold nanozymes toward TMB is significantly higher than their efficiency for the DAB. Besides, the Km value for DAB was found to be very higher than that for TMB. This difference can be related to the different reactivity of DAB and TMB. as well as their different oxidation mechanism.
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