The viability of cell that encapsulated in calcium alginate hydrogel beads-Reference-Cited by-同舟云学术

The viability of cell that encapsulated in calcium alginate hydrogel beads

Published:2022-01-01 Issue:1 Volume:29 Page:473-480
ISSN:2191-0359
Container-title:Science and Engineering of Composite Materials
language:en
Short-container-title:

Author:

Li Fang-Fang¹,Tang Wei-Feng¹,Xie Qiu-Fei²

Affiliation:

1. Third Clinical Division, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials , No.10, Huayuan East Road, Haidian District , Beijing , 100191 , PR China

2. Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials , No.22, Zhongguancun South Avenue, Haidian District , Beijing , 1000

Abstract

Abstract Objective To prove that calcium alginate beads can be used as scaffolds during in vitro culture. Methods Mouse preosteoblastic cells (MC3T3-E1) were encapsulated in calcium alginate hydrogel beads. The Cell Counting Kit-8 (CCK-8) assay was used to assess cell viability at 2, 5, 8, 11, 14, and 21 days. Calcein-AM and propidium iodide (PI) were employed for live/dead staining. Results MC3T3-E1 cells were alive on day 21 and had the highest viability on day 14. Conclusion MC3T3-E1 cells could be encapsulated in calcium alginate hydrogel beads and cultured. Calcium alginate hydrogel beads can be used as scaffolds for three-dimensional in vitro culture.

Publisher

Walter de Gruyter GmbH

Subject

Materials Chemistry,Ceramics and Composites

Link

https://www.degruyter.com/document/doi/10.1515/secm-2022-0156/pdf

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