A New Method to Extract Oridonin and Rosmarinic Acid Simultaneously from Rabdosia Rubescens

Abstract

AbstractA new approach employing conventional solid-liquid extraction has been developed to separate oridonin (ORI) and rosmarinic acid (RA) simultaneously from Rabdosia rubescens. The effects of important parameters were optimized by response surface methodology (RSM) method based on five-level, three-variable central composite design (CCD). A higher recovery rate of 65 % ORI and 88.5 % RA in conjunction with a maximum yield of 9.6 % on the plant dry weight was obtained when liquid-to-solid ratio (LtS) was 12 ml/g, ethanol content (EtC) of 80 %, extract time for 75 min. A quadratic equation was proposed to correlate the independent variables for maximum 9.6 % yield and it is in consistence with the predictive value of 9.52 %. High performance liquid chromatography (HPLC) analysis indicated that ORI and RA in final product were 6.65 times and 9.1 times richer than raw material. With the newly developed process, an extract enriched ORI and RA was obtained with fewer cost and energy.