Affiliation:
1. University of Zanjan , Department of Food Science and Engineering , Zanjan , Iran (Islamic Republic of)
2. Department of Chemical Engineering , Isfahan University of Technology , Isfahan , Iran
Abstract
Abstract
For the first time, the modified supercritical carbon dioxide extraction (MSCE) of biologically active compounds from Feijoa sellowiana leaves was investigated. Total yield (TY) and bioactivity of biocompounds in terms of DPPH• (%DPPHsc) and HO• (%HOsc) radicals scavenging were maximized via multivariate optimization. The optimized conditions were the pressure of 272.34 bar, supercritical carbon dioxide flow rate of 1.58 mL min−1, temperature of 52.48 °C, and dynamic extraction time of 110.62 min. The predicted values of the TY, %DPPHsc, and %HOsc were 81.12 mg g−1, 85.16 %, and 75.66 %, respectively. The chromatographic analysis demonstrated gallic acid as the main biophenolic (136.20 ± 0.16 mg g−1). The scanning electron microscopy images showed dramatic disruption of cells treated with MSCE. A comprehensive comparison was made between current findings and other techniques (conventional and ultrasonic waves assisted extraction). More TY was achieved using conventional method but the bioactivity of MSCE products was considerably better.
Subject
Engineering (miscellaneous),Food Science,Biotechnology
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