Quantitative expression analysis and prognostic significance of the novel apoptosis-related gene BCL2L12 in colon cancer

Author:

Kontos Christos K.,Papadopoulos Iordanis N.,Scorilas Andreas

Abstract

Abstract Apoptosis is a tightly regulated process that plays a critical role in many biological events. Members of the BCL2 (Bcl-2) family of apoptosis-related genes have been found to be differentially expressed in various malignancies and have been proposed as prognostic tumor biomarkers. We have recently discovered and cloned a new member of the BCL2 gene family, BCL2L12, expressed in colon tissues. Here we have investigated expression of the BCL2L12 gene in colon cancer tissues and assessed its prognostic value. Total RNA was isolated from 96 specimens of malignant colon tissue. After testing the RNA quality, cDNA was prepared by reverse transcription. A highly sensitive real-time PCR method for BCL2L12 mRNA quantification was developed using SYBR® Green chemistry. GAPDH served as a housekeeping gene. Relative quantification analysis was performed using the comparative CT method (2-ΔΔCT). High BCL2L12 expression levels were found in smaller (≤5 cm, p=0.027) and well-differentiated tumors (p=0.034), as well as in early-stage tumors (p=0.039). Survival analysis demonstrated that patients with BCL2L12-positive colon tumors have significantly longer disease-free survival and overall survival (p=0.015 and p=0.027, respectively). Our results suggest that BCL2L12 gene expression may represent a potential new biomarker for colon cancer.

Publisher

Walter de Gruyter GmbH

Subject

Clinical Biochemistry,Molecular Biology,Biochemistry

Reference27 articles.

1. Breast cancer cells response to the antineoplastic agents cisplatin carboplatin and doxorubicin at the mRNA expression levels of distinct apoptosis - related genes , including the new member;Thomadaki;Ann NY Acad Sci,2007

2. Tumour markers of prog - nosis in colorectal cancer;McLeod;Cancer,1999

3. gene family and related proteins in mammary gland involution and breast cancer;Schorr;J Mammary Gland Biol Neoplasia,1999

4. Molecular cloning physical mapping , and expression analysis of a novel gene encoding a proline - rich protein with a highly con - served BH domain of the Bcl - family;Scorilas;Genomics,2001

5. High expression of Bcl - protein in acute myeloid leukemia cells is associated with poor response to chemo - therapy;Tothova;Neoplasma,2002

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3