Genetic Diversity and Population Structure of Apricot (Prunus armeniaca L.) from Northern Pakistan using Simple Sequence Repeats

Abstract

Abstract A collection of 44 P. armeniaca accessions and three related species, from diverse geographic areas, covering the entire Northern hemisphere with emphasis on Pakistani apricots, was screened with 10 SSR primer pairs developed in apricot, to characterize the cultivars and establish their genetic relationship. Given the fact that the Central Asian region is considered a center of origin of apricot, particular attention was devoted to accessions from the Hunza region of Pakistan. The primers correctly amplified a repeatable polymorphic pattern, which unequivocally distinguished all genotypes under study. Altogether 123 alleles were identified with an average of 12.30 alleles per locus. The observed heterozygosity for individual loci ranged from 0.28 to 0.77 with an average of 0.64. A neighbour joining method identified four groups from: (A) Central Asia, (B) Irano-Caucasia, (C) Continental Europe and (D) North America. The dendrogram confirms the historic dissemination pathways of apricot from its centre of origin in Asia to the West. Apricot cultivars from the Hunza region (Northern Pakistan) revealed a high variability, as genetic diversity is still conserved due to the traditional practice of planting seeds from the best trees.