Association of bitter and sweet taste gene receptor polymorphisms with dental caries formation
Author:
Yilmaz Melis1ORCID, Balci Senay2ORCID, Kocak Topbas Nazan3ORCID, Derici Yildirim Didem4ORCID, Tamer Lulufer2ORCID
Affiliation:
1. Department of Endodontics , School of Dentistry, Mersin University , Mersin , Turkey 2. Department Medical Biochemistry , Faculty of Medicine, Mersin University , Mersin , Turkey 3. Department of Oral and Maxillofacial Radiology , School of Dentistry, Mersin University , Mersin , Turkey 4. Biostatistics and Medical Informatics Department , Faculty of Medicine, Mersin University , Mersin , Turkey
Abstract
Abstract
Objectives
The aim of the study is to analyze the association of different bitter and sweet gene receptor polymorphisms and bitter and sweet food consumption on formation of dental caries in Turkish adult population.
Methods
This study included 205 adults whose detailed intraoral health examination was completed and decayed, missing, filled teeth (DMFT) index values were recorded. A mini questionnaire was applied to assess the consumption of bitter and sweet food. A venous blood sample from each participant was collected in Ethylenediamine tetraacetic acid (EDTA) tubes. Further, DNA samples were isolated from the blood samples by utilizing a DNA isolation kit, which were stored at +4 °C prior to the analysis. Taste receptor type 2 member 38 (TAS2R38; rs10246939, rs713598, rs1726866), Taste receptor type 1 member 2 (TAS1R2; rs35874116, rs9701796), and Taste receptor type 1 member 3 (TAS1R3; rs307355) gene polymorphisms were detected using real-time polymerase chain reaction (PCR).
Results
There was no statistically significant association between the TAS2R38, TAS1R2, and TAS1R3 gene polymorphism and the DMFT index (p>0.05). No significant difference was found between the consumption of bitter and sweet food and the DMFT index (p>0.05).
Conclusions
TAS2R38 (rs10246939, rs713598, rs1726866), TAS1R2 (rs35874116, rs9701796), and TAS1R3 (rs307355) gene polymorphism may not be associated with the formation of dental caries in the Turkish adult population.
Publisher
Walter de Gruyter GmbH
Subject
Biochemistry (medical),Clinical Biochemistry,Molecular Biology,Biochemistry
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