Preparation, characterization, and stability of lipid nanoparticles including unsaturated lipids
Author:
Park Yeseul1, Park Jin-Won1
Affiliation:
1. Department of Chemical and Biomolecular Engineering, College of Energy and Biotechnology , Seoul National University of Science and Technology , Seoul , Republic of Korea
Abstract
Abstract
Among the SARS-CoV-2 vaccines developed to date, the mRNA vaccines developed by the Pfizer/BioNTech and Moderna companies have been formulated using saturated lipids, specifically 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), along with cholesterol. DSPC and cholesterol have the disadvantage of causing sclerosis. Therefore, in this study, lipid nanoparticles (LNPs) were prepared and characterized by replacing DSPC with 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and excluding cholesterol. The results showed that the DOPC-based LNPs had a smaller diameter (75.67 nm) compared to the previous study and the polydispersity index (PI) indicated a good dispersion homogeneity, suggesting size uniformity. Additionally, the LNPs maintained their size between 57 nm and 152 nm and showed stable PI values (0.330–0.393) throughout the 25 days.
Publisher
Walter de Gruyter GmbH
Subject
Condensed Matter Physics,General Chemical Engineering,General Chemistry
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