Use of Immobilized Candida Cells on Xylitol Production from Sugarcane Bagasse

Author:

Carvalho Walter de1,Silva Silvio Silvério da2,Vitolo Michele3,Mancilha Ismael Maciel de1

Affiliation:

1. Department of Biotechnology, Faculty of Chemical Engineering of Lorena, Lorena, SP, 12600-000, Brazil.

2. Department of Biotechnology, Faculty of Chemical Engineering of Lorena, Lorena, SP, 12600-000, Brazil. Fax: (+55) 1 25533165.

3. Faculty of Pharmaceutical Sciences, University of Säo Paulo, Säo Paulo, SP, 05489-900, Brazil

Abstract

Abstract In this study we used the yeast Candida guilliermondii FTI 20037 immobilized by entrapment in Ca-alginate beads (2 .5 -3 mm diameter) for xylitol production from concentrated sugarcane bagasse hemicellulosic hydrolysate in a repeated batch system. The fermentation runs were carried out in 125- and 250-ml Erlenmeyer flasks placed in an orbital shaker at 30 °C and 200 rpm during 72 h, keeping constant the proportion between work volume and flask total volume. According to the results, cell viability was substantially high (98%) in all fermentative cycles. The values of parameters xylitol yield and volumetric productivity increased significantly with the reutilization of the immobilized biocatalysts. The highest values of xylitol final concentration (11.05 g/1), yield factor (0.47 gig) and volumetric productivity (0.22 g/lh) were obtained in 250-ml Erlenmeyer flasks containing 80 ml of medium plus 20 mi of immobilized biocatalysts. The support used in this study (Ca-alginate) presented stability in the experimental conditions used. The results show that the use of immobilized cells is a promising approach for increasing the xylitol production rates.

Publisher

Walter de Gruyter GmbH

Subject

General Biochemistry, Genetics and Molecular Biology

Cited by 13 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Immobilization of recombinant Escherichia coli on multi-walled carbon nanotubes for xylitol production;Enzyme and Microbial Technology;2020-04

2. Xylitol production in immobilized cultures: a recent review;Critical Reviews in Biotechnology;2015-02-10

3. Production of Extracellular Isoamylase and Endoglucanase by Entrapped Spores of Rhizopus oryzae PR7;Proceedings of the National Academy of Sciences, India Section B: Biological Sciences;2014-09-12

4. A review on different modes and methods for yielding a pentose sugar: xylitol;International Journal of Food Sciences and Nutrition;2013-10-25

5. Fermentation Strategies Explored for Xylitol Production;D-Xylitol;2012

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