Phytochemical analysis, antioxidant, cytotoxic, and antimicrobial activities of golden chamomile (Matricaria aurea (Loefl.) Schultz Bip)
Author:
Yousefbeyk Fatemeh1, Hemmati Ghazaleh1, Gholipour Ziba1, Ghasemi Saeed2, Evazalipour Mehdi3, Schubert Clara4, Koohi Diba E.1, Böhm Volker4ORCID
Affiliation:
1. Department of Pharmacognosy , School of Pharmacy, Guilan University of Medical Sciences , Rasht , Iran 2. Department of Medicinal Chemistry , School of Pharmacy, Guilan University of Medical Sciences , Rasht , Iran 3. Department of Pharmaceutical Biotechnology , School of Pharmacy, Guilan University of Medical Sciences , Rasht , Iran 4. Institute of Nutritional Sciences, Friedrich Schiller University Jena , Jena , Germany
Abstract
Abstract
Matricaria aurea (Loefl.) Schultz Bip. (Asteraceae), known as golden chamomile, has been traditionally used for the treatment of various diseases. In this study, total phenolic, flavonoid, and tannin contents of total extract and different fractions of this plant were determined. The antioxidant, cytotoxic, and antimicrobial activities were also evaluated. Moreover, the phenolic profiles of selected fractions were determined by HPLC and LC-MS/MS analysis. Results demonstrated total phenolic contents of 37.8–57.2 mg GAE/g and total flavonoid contents of 3.0–111.2 mg QE/g. The ethyl acetate and methanol fractions (EF and MF) had the highest concentrations of phenolic, tannin, and flavonoid compounds. In both DPPH radical scavenging assay and phosphomolybdenum reduction assay, EF showed the best antioxidant activity, followed by MF. EF and MF indicated also the best antibacterial activities against Bacillus subtilis (MIC 1.56 and 12.5 mg ml−1) and Staphylococcus aureus (MIC 0.78 and 12.5 mg ml−1). Hexane fraction (HF) had no antibacterial effect. None of the samples had antifungal effect. MTT (3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay revealed for EF and HF the highest antiproliferative activities (IC50 values ranged from 111.8 to 294.6 μg ml−1). The presence of chlorogenic acid, ferulic acid, and luteolin-7-O-glucoside in MF, and p-coumaric acid in EF was confirmed and quantified.
Funder
Guilan University of Medical Sciences
Publisher
Walter de Gruyter GmbH
Subject
General Biochemistry, Genetics and Molecular Biology
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