Two-photon fluorescent turn-on probes for highly efficient detection and profiling of thiols in live cells and tissues-Reference-Cited by-同舟云学术

Two-photon fluorescent turn-on probes for highly efficient detection and profiling of thiols in live cells and tissues

Published:2021-09-09 Issue:0 Volume:0 Page:
ISSN:1431-6730
Container-title:Biological Chemistry
language:en
Short-container-title:

Author:

Shen Congzhen¹,Zhang Duoteng²,Xu Fang¹,Yang Yang³^ORCID,Tan Yi¹,Zhao Qian³,Li Lin²,Ding Ke¹,Li Zhengqiu¹⁴

Affiliation:

1. School of Pharmacy, Jinan University , 601 Huangpu Avenue West , Guangzhou 510632 , China

2. Key Laboratory of Flexible Electronics (KLOFE), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech) , 30 South Puzhu Road , Nanjing 211800 , China

3. Department of Applied Biology and Chemical Technology , State Key Laboratory of Chemical Biology and Drug Discovery, Hong Kong Polytechnic University , Hong Kong , China

4. MOE Key Laboratory of Tumor Molecular Biology, Jinan University , Guangzhou , China

Abstract

Abstract Thiols are important units in amino acids such as cysteine and peptides like glutathione. Development of chemical sensors capable of precise detection of thiols is important in cancer diagnosis and therapy. We have developed novel two-photon fluorescent turn-on probes for selective detection of thiols. The probes displayed excellent sensitivity and low detection limits. The dual-purpose probes have been demonstrated to be suitable for simultaneous imaging and proteome profiling in live cells and tumor tissues. The unique turn-on design endows the probes with excellent selectivity toward thiols in vitro and in situ, and can be further developed to support a thiol-quantification assay.

Publisher

Walter de Gruyter GmbH

Subject

Clinical Biochemistry,Molecular Biology,Biochemistry

Link

https://www.degruyter.com/document/doi/10.1515/hsz-2021-0189/pdf

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