Affiliation:
1. Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Universität Düsseldorf, Universitätsstraße 1, D-4000 Düsseldorf, Bundesrepublik Deutschland
Abstract
Rosmarinic acid biosynthesis can be stimulated in cell cultures of Coleus blumei by culturing the cells in medium with 4% sucrose. In enzyme extracts of these cells two new enzymes of rosmarinic acid biosynthesis were discovered. Hydroxyphenylpyruvate reductase reduces 4-hydroxyphenylpyruvate and 3,4-dihydroxyphenylpyruvate to 4-hydroxyphenyllactate and 3,4-dihydroxyphenyllactate, respectively, using NADH. Rosmarinic acid synthase transfers the caffeoyl moiety of caffeoyl-CoA to the non-phenolic OH-group of 3,4-dihydroxyphenyllactic acid, in course of which rosmarinic acid is formed.
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
67 articles.
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