Optimisation of a multiplex PCR assay of nuclear microsatellite markers for population genetics and clone identification in Robinia pseudoacacia L.-Reference-Cited by-同舟云学术

Optimisation of a multiplex PCR assay of nuclear microsatellite markers for population genetics and clone identification in Robinia pseudoacacia L.

Published:2012-12-01 Issue:1-6 Volume:61 Page:142-148
ISSN:2509-8934
Container-title:Silvae Genetica
language:en
Short-container-title:

Author:

Liesebach H.¹,Ewald E.²

Affiliation:

1. Thünen Institute of Forest Genetics, Sieker Landstraße 2, D-22927 Grosshansdorf , Germany

2. Thünen Institute of Forest Genetics, Eberswalder Chaussee 3A, D-15377 Waldsieversdorf , Germany

Abstract

Abstract Black locust (Robinia pseudoacacia L.) is a tree species native to North America. The multipurpose tree is cultivated worldwide, but causes problems due to its partially invasive character. The application of nuclear microsatellite loci has many aims in population genetic studies. Here we introduce a very cost-effective method for combining the information of 14 nuclear microsatellite loci into two multiplex PCR sets as a contribution to greater standardisation and more comparable results. Combined non-exclusion probabilities for clone identification using example populations are estimated at between 1.37*E-5 and 1.67*E-11, and for paternity analysis for 1.59*E-4. The detected weak linkage between some microsatellite loci is not considered to be a substantial restriction to the reliability of the set of markers in providing an appropriate method for fingerprinting and parentage analysis.

Publisher

Walter de Gruyter GmbH

Subject

Genetics,Forestry

Reference35 articles.

1. AMOS, W., J. I. HOFFMAN, A. FRODSHAM, L. ZHANG, S. BEST and A. V. S. HILL (2007): Automated binning of microsatellite alleles: problems and solutions. Molecular Ecology Notes 7: 10-14.

2. BÖCKER, R. and M. DIRK (1998): Distribution and Spreading of Alien Trees and Shrubs in South Western Germany and Contributions to Germination Biology. pp. 285-297. In: Plant Invasions: Ecological Mechanisms and Human Responses, edited by U. STARFINGER, K. EDWARDS, I. KOWARIK and M. WILLIAMSON, Backhuys Publishers, Leiden, The Netherlands.

3. BÖHMER, H. J., T. HEGER and L. TREPL (2001): Robinia pseudoacacia L., Black locust. pp. 64-75. In: Fall - studien zu gebietsfremden Arten in Deutschland - Case Studies on Alien Species in Germany, Umweltbundesamt (Federal Environment Agency), Berlin.

4. BONGARTEN, B. C., D. A. HUBER and D. K. APSLEY (1992): Environmental and genetic influences on short-rotation biomass production of black locust (Robinia pseudoacacia L.) in the Georgia Piedmont. Forest Ecology and Management 55: 315-331.

5. BROOKFIELD, J. F. Y. (1996): A simple new method for estimating null allele frequency from heterozygote deficiency. Molecular Ecology 5: 453-455.

Cited by 6 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Analysis of the genetic diversity and population structures of black locust (Robinia pseudoacacia L.) stands in Poland based on simple sequence repeat markers;Folia Forestalia Polonica;2023-12-01

2. Development and Application of EST-SSR Markers for DNA Fingerprinting and Genetic Diversity Analysis of the Main Cultivars of Black Locust (Robinia pseudoacacia L.) in China;Forests;2019-07-30

3. Evaluation of the Genetic Diversity and Differentiation of Black Locust (Robinia pseudoacacia L.) Based on Genomic and Expressed Sequence Tag-Simple Sequence Repeats;International Journal of Molecular Sciences;2018-08-23

4. Züchtung, Zulassungen, Vermehrung;Agrarholz – Schnellwachsende Bäume in der Landwirtschaft;2018

5. Clone Identification among Saplings in a Natural Forest of Thujopsis dolabrata var. hondae Using Multiplex EST-SSR Analysis;Journal of the Japanese Forest Society;2015