Affiliation:
1. College of Food Science and Technology , 70573 Henan Agricultural University , Zhengzhou 450002 , China
2. Institute of Microbiology , Guangdong Academy of Sciences , Guangzhou , China
Abstract
Abstract
The integration of β-carotene into functional foods is limited due to its limited hydrophilicity and chemical stability. To address this issue, emulsion was encouraged to be utilized. Monolayer, composite, and bilayer emulsions were prepared using pea protein isolate (PPI) alone or in combination with poly-γ-glutamic acid (γ-PGA). Our findings indicate that the bilayer emulsion utilizing γ-PGA as an external emulsifier exhibited the smallest droplet size (737.61 nm) and highest zeta potential (−35.23 mV). Under various environmental stresses, the chemical stability of the composite emulsion surpassed that of the monolayer emulsion but was inferior to that of the bilayer emulsion. Moreover, following simulated gastrointestinal digestion, the β-carotene bioaccessibility in the composite emulsion (12.76 %) exceeded that in the monolayer emulsion (6.57 %) but was less than that in the bilayer emulsion (20.01 %). Therefore, the emulsion preparation method or the sequence of γ-PGA addition significantly affects emulsion stability. Among the studied emulsions, the bilayer emulsion demonstrated the most stable structure, enhancing the stability of β-carotene within the oil droplets.
Funder
the Natural Science Foundation of Henan
the Key Research and Development Projects in Henan Province
the 2023 Henan Provincial County (City) Innovation Guidance Plan
Jiaozuo Science and Technology Commissioner Service Team
the Key Scientific and Technological Project of Henan Provincial Education Department of China
nnovative Intelligence Development Project