Chemical stability of β-carotene composite or bilayer emulsions coated with pea protein isolate and γ-polyglutamic acid compared to monolayer emulsion

Author:

Zhang Bobo1ORCID,Zhu Hongshuai1,Xie Xinhua1,Shi Qingshan2,Liang Dan1,Chen Kai1

Affiliation:

1. College of Food Science and Technology , 70573 Henan Agricultural University , Zhengzhou 450002 , China

2. Institute of Microbiology , Guangdong Academy of Sciences , Guangzhou , China

Abstract

Abstract The integration of β-carotene into functional foods is limited due to its limited hydrophilicity and chemical stability. To address this issue, emulsion was encouraged to be utilized. Monolayer, composite, and bilayer emulsions were prepared using pea protein isolate (PPI) alone or in combination with poly-γ-glutamic acid (γ-PGA). Our findings indicate that the bilayer emulsion utilizing γ-PGA as an external emulsifier exhibited the smallest droplet size (737.61 nm) and highest zeta potential (−35.23 mV). Under various environmental stresses, the chemical stability of the composite emulsion surpassed that of the monolayer emulsion but was inferior to that of the bilayer emulsion. Moreover, following simulated gastrointestinal digestion, the β-carotene bioaccessibility in the composite emulsion (12.76 %) exceeded that in the monolayer emulsion (6.57 %) but was less than that in the bilayer emulsion (20.01 %). Therefore, the emulsion preparation method or the sequence of γ-PGA addition significantly affects emulsion stability. Among the studied emulsions, the bilayer emulsion demonstrated the most stable structure, enhancing the stability of β-carotene within the oil droplets.

Funder

the Natural Science Foundation of Henan

the Key Research and Development Projects in Henan Province

the 2023 Henan Provincial County (City) Innovation Guidance Plan

Jiaozuo Science and Technology Commissioner Service Team

the Key Scientific and Technological Project of Henan Provincial Education Department of China

nnovative Intelligence Development Project

Publisher

Walter de Gruyter GmbH

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