Characterisation of compound middle lamella isolated by a combination of wet-beating, sedimentation, and methanol dialysis
Author:
Matsushita Yasuyuki1, Fukumura Taichi1, Aoki Dan1, Fukushima Kazuhiko1
Affiliation:
1. Graduate School of Bioagricultural Sciences , Nagoya University , Nagoya 464-8601 , Japan
Abstract
Abstract
A simple method was developed to isolate the compound middle lamella (CML) in its natural state using wet-beating, sedimentation, and methanol dialysis. The isolated CML fraction was characterised. The CML was isolated in 2.0% yield from the mature wood of Ginkgo biloba. Scanning electron microscopy (SEM) images of the isolated CML fraction showed the presence of flake-like structures. However, some small amounts of secondary wall substance was observed. The lignin content of the CML fraction was approximately 45%. Thioacidolysis reactions with the isolated CML fraction revealed that the lignin β-O-4 structure was scarce in the isolated fraction and that numerous structures with two or more consecutive condensed-type bonds were present. Results from the solid-state carbon-13 nuclear magnetic resonance (13C NMR) experiments indicated that the CML fraction had low crystallinity, indicating low cellulose content. Liquid-state NMR analysis of the lignin from the CML fraction revealed the absence of p-hydroxyphenyl (H) units.
Publisher
Walter de Gruyter GmbH
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