Evaluation of ring-5 structures of guaiacyl lignin in Ginkgo biloba L. using solid- and liquid-state 13C NMR difference spectroscopy
Author:
Aoki Dan1, Nomura Kenta1, Hashiura Masashi1, Imamura Yoshinori1, Miyata Sonoka1, Terashima Noritsugu2, Matsushita Yasuyuki1, Nishimura Hiroshi3, Watanabe Takashi3, Katahira Masato4, Fukushima Kazuhiko1
Affiliation:
1. Graduate School of Bioagricultural Sciences , Nagoya University , Nagoya 464-8601 , Japan 2. Nagoya University , 2-610 Uedayama, Tenpaku , Nagoya 468-0001 , Japan 3. Research Institute for Sustainable Humanosphere , Kyoto University , Uji 611-0011 , Japan 4. Institute of Advanced Energy (IAE) , Kyoto University , Uji 611-0011 , Japan
Abstract
Abstract
To discuss the macromolecular structure and properties of lignin, the complementary use of solid- and liquid-state nuclear magnetic resonance (NMR) combined with a specific 13C-enrichment technique may provide useful information. The 13C-enriched lignin was prepared by administering [guaiacyl ring-5(G5)-13C]-coniferin to a growing Ginkgo biloba L. shoot. The 13C-enriched cellulolytic enzyme lignin (EL) and its acetate prepared from the ginkgo shoot were examined by solid- and liquid-state 13C NMR spectroscopy. The 13C NMR spectrum derived only from the G5 carbon was obtained as a difference spectrum based on the spectra of the G5-13C enriched and un-enriched (UE) samples. The condensed structures, including the enriched G5-carbon, were evaluated using difference spectra. The chemical shifts of the enriched G5 carbon in both the solid- and liquid-state 13C NMR spectra agreed with each other in principle. The quantitative ratio of the condensed and uncondensed structures at G5 was found to be larger by solid-state cross polarization/magic angle spinning (CP/MAS) NMR than by liquid-state NMR.
Funder
Japan Society for the Promotion of Science KAKENHI
Publisher
Walter de Gruyter GmbH
Reference54 articles.
1. Alves, V.L., Drumond, M.G., Stefani, G.M., Chen, C.-L., Piló-Veloso, D. (2000) Synthesis of new trimeric lignin model compounds containing 5-5′ and β-O-4′ substructures, and their characterization by 1D and 2D NMR techniques. J. Braz. Chem. Soc. 11:467–473. 2. Aoki, D., Hanaya, Y., Akita, T., Matsushita, Y., Yoshida, M., Kuroda, K., Yagami, S., Takama, R., Fukushima, K. (2016) Distribution of coniferin in freeze-fixed stem of Ginkgo biloba L. by cryo-TOF-SIMS/SEM. Sci. Rep. 6:31525. 3. Atalla, R.H., Gast, J.C., Sindorf, D.W., Bartuska, V.J., Maciel, G.E. (1980) 13C NMR spectra of cellulose polymorphs. J. Am. Chem. Soc. 102:3249–3251. 4. Bardet, M., Gerbaud, G., Trân, Q.-K., Hediger, S. (2007) Study of interactions between polyethylene glycol and archaeological wood components by 13C high-resolution solid-state CP/MAS NMR. J. Archaeol. Sci. 34:1670–1676. 5. Campbell, G.C., Crosby, R.C., Haw, J.F. (1986) 13C chemical shifts which obey the curie law in CP/MAS NMR spectra. The first CP/MAS NMR chemical-shift thermometer. J. Magn. Reson. 69:191–195.
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