TK-PUL, a pullulan hydrolase type III from Thermococcus kodakarensis, a potential candidate for simultaneous liquefaction and saccharification of starch-Reference-Cited by-同舟云学术

TK-PUL, a pullulan hydrolase type III from Thermococcus kodakarensis, a potential candidate for simultaneous liquefaction and saccharification of starch

Published:2020-01-01 Issue:1 Volume:4 Page:45-55
ISSN:2450-9728
Container-title:Amylase
language:en
Short-container-title:

Author:

Toor Khurram Jahangir¹,Ahmad Nasir¹,Muhammad Majida Atta¹,Rashid Naeem²

Affiliation:

1. School of Biological Sciences , University of the Punjab , Quaid-e-Azam Campus, Lahore 54590 , Pakistan

2. School of Biological Sciences , University of the Punjab , Quaid-e-Azam Campus, Lahore 54590 , Pakistan , E-mail:naeem.ff.sbs@pu.edu.pk

Abstract

Abstract TK-PUL, a novel thermo-acidophilic pullulanase from Thermococcus kodakarensis and a unique member of glycoside hydrolase family GH13 was successfully produced in Escherichia coli grown by fed batch culture in a fermenter and partially purified by simple heat treatment. Specific activity of partially purified TK-PUL was 28 U/mg. Corn starch was successfully liquefied and saccharified using this single enzyme at pH 4.2. Simultaneous liquefaction and saccharification of corn starch by TK-PUL was comparable to Termamyl, a commercially available starch-hydrolyzing industrial enzyme. Both enzymes efficiently hydrolysed corn starch into sugar syrups having major proportions of maltose. TK-PUL performs efficiently at the natural pH of starch (~4.5) in the absence of any metal ions, hence is a potential candidate for starch industry.

Publisher

Walter de Gruyter GmbH

Link

https://www.degruyter.com/document/doi/10.1515/amylase-2020-0004/pdf

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