Increased Activity of Hippocampal Antioxidant Enzymes as an Important Adaptive Phenomenon of the Antioxidant Defense System in Chronically Stressed Rats

Author:

Popović Nataša1,Pajović B. Snežana1,Stojiljković Vesna1,Todorović Ana1,Pejić Snežana1,Pavlović Ivan1,Gavrilović Ljubica1

Affiliation:

1. Institute of Nuclear Sciences “Vinča”, Laboratory of Molecular Biology and Endocrinology, University of Belgrade, Belgrade , Serbia

Abstract

Abstract This study examined the effects of chronic restraint stress (CRS: 2 hours × 14 days) on gene expression of three antioxidant enzymes, copper, zinc superoxide dismutase (SOD 1), manganese superoxide dismutase (SOD 2) and catalase (CAT) in the rat hippocampus. Also, we examined changes in the activities of SOD 1, SOD 2 and CAT in the hippocampus of chronically stressed rats. Investigated parameters were quantifi ed by using real-time RT-PCR, Western blot analysis and assay of enzymatic activity. We found that CRS did not change mRNA and protein levels of SOD 1 and CAT, but increased mRNA and protein levels of SOD 2. However, CRS treatment increased the enzyme activities of SOD 1, SOD 2 and CAT. Our fi ndings indicate that the increased activity of antioxidant enzymes (SOD 1, SOD 2 and CAT) in the hippocampus may be an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats.

Publisher

Walter de Gruyter GmbH

Subject

General Veterinary

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