Isolation and purification of 12 flavonoid glycosides from <i>Ginkgo biloba</i> extract using sephadex LH-20 and preparative high-performance liquid chromatography-Reference-Cited by-同舟云学术

Isolation and purification of 12 flavonoid glycosides from Ginkgo biloba extract using sephadex LH-20 and preparative high-performance liquid chromatography

Published:2022-11-10 Issue:1-2 Volume:78 Page:73-81
ISSN:0939-5075
Container-title:Zeitschrift für Naturforschung C
language:en
Short-container-title:

Author:

Li Ping¹,Sun Hao¹²,Li Wei³,Wu Qi⁴,Ye Shuhong¹,Zhu Jingbo¹⁵,Ding Yan¹

Affiliation:

1. School of Food Science and Technology, Dalian Polytechnic University , Dalian , Liaoning , , China

2. Department of Chemistry , Inha University , 100 Inharo , Incheon , , Korea

3. Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine , Daegu , , South Korea

4. China National Institute of Standardization , Beijing , , China

5. Institute of Chemistry and Applications of Plant Resources, Dalian Polytechnic University , Dalian , Liaoning , , China

Abstract

Abstract An efficient and rapid preparative method for the separation and purification of flavonoid glycosides from the Ginkgo biloba extract (GBE) was developed by sephadex LH-20 and preparative high-performance liquid chromatography (HPLC). 40 g GBE of 24% flavonoids were loaded onto the sephadex LH-20 column and five fractions (1.15, 2.57, 1.32, 4.45, and 3.31 g) at flavonoid content of 72.3, 54.2, 63.5, 51.2, and 59.2% were produced. Ultimately, 12 flavonoid glycosides that are at least purities of 97.7% were obtained from 100 mg of each fraction by preparative HPLC. The fraction A, B, and D each contained two flavonoids, yielded 35, 30, 23, 20, 25, and 25 mg, respectively. The fraction C and E each contained three flavonoids, produced 20, 13, 15, 18, 15, and 20 mg, respectively. The chemical structures of the purified compounds were identified by nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI/MS).

Funder

Natural Science Foundation of Liaoning Province

National Natural Science Foundation of China

Publisher

Walter de Gruyter GmbH

Subject

General Biochemistry, Genetics and Molecular Biology

Link

https://www.degruyter.com/document/doi/10.1515/znc-2022-0124/pdf

Reference30 articles.

1. Zhang, CW, Wang, CZ, Tao, R, Fang, CJ. Enzymolysis-based ultrasound extraction and antioxidant activities of polyprenol lipids from Ginkgo biloba leaves. Process Biochem 2016;51:444–51. https://doi.org/10.1016/j.procbio.2015.12.008.

2. Boateng, ID, Yang, XM. Ginkgo biloba L. seed; A comprehensive review of bioactives, toxicants, and processing effects. Ind Crops Prod 2gft022;176:114281. https://doi.org/10.1016/j.indcrop.2021.114281.

3. Zhang, XH, Sun, ZY, Cao, FL, Ahmad, H, Yang, XH, Zhao, LG, et al.. Effects of dietary supplementation with fermented Ginkgo leaves on antioxidant capacity, intestinal morphology and microbial ecology in broiler chicks. Br Poultry Sci 2015;56:370–80. https://doi.org/10.1080/00071668.2015.1030590.

4. Wang, HY, Zhang, YQ. The main active constituents and detoxification process of Ginkgo biloba seeds and their potential use in functional health foods. J Food Compos Anal 2018;83:103247. https://doi.org/10.1016/j.jfca.2019.103247.

5. Kim, MS, Bang, JH, Lee, J, Han, JS, Bailk, TG, Jeon, WK. Ginkgo biloba L. extract protects against chronic cerebral hypoperfusion by modulating neuroinflammation and the cholinergic system. Phytomedicine 2016;23:1356–64. https://doi.org/10.1016/j.phymed.2016.07.013.