Partial purification, characterization and wheat bran degradation studies of a new phytase from the Bacillus megaterium EBD 9-1 strain

Abstract

AbstractObjective:The present study was designed to report the bacterial identification and characterization of a new phytase enzyme from aMethods:sp. strain was identified based on 16S rRNA analysis. The phytase was partially purified through ammonium sulfate precipitation and Sephadex G100 gel filtration steps, and characterized for its activity and stability.Results:The new isolate EBD 9-1 showed 100% sequence identity withConclusion:Consequently, due to the characteristics such as significant stability at higher temperatures, alkaline pH and storage of the novel phytase enzyme produced by