Phoenix dactylifera Linn fruit based-diets palliate hyperglycemia in alloxan-induced diabetic rats

Abstract

AbstractObjectivesThis study was designed to examine thein vitroinhibitory activities of vital enzymes related to diabetes mellitus and different biochemical parameters ofPhoenix dactyliferafruit based-diet in alloxan-induced diabetic rats.MethodsThe aqueous extract ofP. dactyliferafruit based-diet was prepared and used for determination ofin vitroantioxidants as well as α-amylase and α-glucosidase inhibitory activities using standard procedures. Also, 30 albino rats were induced by a single intraperitoneal injection of 150 mg/kg body weight of alloxan and grouped into A–D as normal rats placed onDioscorea rotundatabased-diet, diabetic control rats placed onD. rotundatabased-diet, diabetic rats placed onD. rotundatabased-diet and administered metformin orally per day, and diabetic rats placed onP. dactyliferafruit based-diet respectively. The animals were sacrificed on the fourth week of the experiment, and different biochemical parameters were evaluated.ResultsTheP. dactyliferafruit based-diet extract demonstrated antioxidative potentials and inhibition against α-amylase and α-glucosidase activities in a dose-dependent manner. In addition, diabetic rats placed on theP. dactyliferafruit based-diet revealed significant (p<0.05) increase in body weight, insulin and glycogen levels, antioxidant enzyme activities, GLUT 2 and high density lipoprotein (HDL) concentrations when compared with the diabetic control group. Also, diabetic rats placed onP. dactyliferafruit based-diet indicate significant (p<0.05) reduction in fasting blood glucose, lipid peroxidation, cytokines levels, some gluconeogenesis enzyme activities, cholesterol, triglycerides, low-density lipoproteins and very low-density lipoproteins concentrations compared to the diabetic control animals.ConclusionThis diet could be an alternative nutraceutical means of managing diabetes mellitus and its complications.