Comparison of Stopped Flow and Steady State Kinetics in the Reaction of D-Amino-Acid Oxidase with β-Chloroalanine

Abstract

We describe anaerobic stopped-flow monitored interactions of D-B-chloroalanine with D-amino acid oxidase and show that the kinetics of absorbance changes at 550 nm due to enzyme-bound intermediates can not be correlated with steady state turnover behavior unless it is assumed that only a small fraction of the enzyme directly participates in the α-β elimination process.