Comparison of spectrophotometric methods of determining Fe(III) using a computer-controlled SuperScanR 3 UV-VIS spectrophotometer

Abstract

Methods of the direct determination of Fe(III) with Eriochromazurol B, Chromazurol S, Eriochromcyanine R, 4-(2-pyridylazo)resorcinol and 4-(2-thiazolylazo)resorcinol were compared using basic parameters for the spectrophotometric methods. The most useful reagents for determining Fe(III) in blood serum are Chromazurol S and PAR. The possibility of direct recording and treatment of the calibration curve using the SuperScanR 3 spectrophotometer controlled by an HP 9815A desk-top calculator is demonstrated.