Comparison of the reactions of ferric ions with Chromazurol S, Eriochromazurol B and Eriochromcyanine R in the presence of surface active substances and spectrophotometric determination of Fe(III)-ions with Chromazurol S-cetylpyridinium bromide system

Abstract

The spectrophotometric methods of determining Fe(III) ions with Chromazurol S, Eriochromazurol B and Eriochromcyanine R in the presence of cationic, anionic and nonionic tensides were compared using the basic optical and statistical parameters. A method was proposed and developed for the determination of Fe(III) ions using Chromazurol S in blood serum and plasma at pH 4.7-5.0 in a medium of 2 . 10-4M cetylpyridinium bromide and 0.1M pyridine buffer and at pH 6.0-6.5 in a medium of 2 . 10-3M cetylpyridinium bromide and 0.1M hexamethylenetetramine buffer with molar absorption coefficients of 1.31 . 105 and 1.40 . 105, respectively. The determination is sufficiently specific even at pathological concentrations of the other serum components.