Study the CAT-21 A/T (rs7943316) gene polymorphism and its correlation with physiological antioxidant index in the Iraqi patients with T2DM

Abstract

Background: A multi-gene inherited metabolic illness in which the body is unable to produce enough insulin and is characterized by abnormal glucose homeostasis has been now called Type 2 diabetes mellitus (T2DM). Objectives: This study aimed to evaluate the effect of CAT-21 A/T (rs7943316) gene polymorphism and antioxidant index (AOI) in the Iraqi patients with T2DM. Methods: In general, 135 individuals, 90 T2DM patients and 45 healthy control were employed in this research. The spectrophotometer method were used to assessment of  TAO-C and MDA levels (antioxidant index TAO-C/MDA), while CAT-21 A/T (rs7943316) SNPs was an estimate by PCR-RFLP and restriction fragments by Hinf1 enzyme restriction.  Results: In this study, the results propose profoundly tremendous contrasts in TAO-C, MDA, and AOI levels among T2DM and the benchmark group (p-value< 0.05). Then again, the outcomes propose the profoundly bad relationship (r=-0.765) between the periods of the T2DM bunch with TAO-C as well as, we tracked down an exceptionally certain connection (r=0.556) between the times of the T2DM bunch with MDA levels. Furthermore, in the genetic part of this work, the results indicated that the AA homozygote genotype implied a statistically significant effect (P-value=0.000) (OR=2.66(1.23-4.12)) for the risk of T2DM.