Protective effect of aqueous extract of Nigella sativa on Oxidative Enzymes, Homocysteine, and Lipids in Methionine induced Hyperhomocysteinemic rats

Abstract

Hyperhomocysteinemia has emerged as an independent risk factor for development of various diseases such as coronary, cerebrovascular and peripheral arterial occlusive diseases. Its association is found with atherosclerosis, cancer and some other aged-related illnesses including Alzheimer’s disease. The present study was designed to investigate the homocysteine lowering potential, effect on lipids and oxidative enzymes of standardized aqueous extract of Nigella sativa seeds (100 and 200 mg/kg body weight, p.o.) in hyperhomocysteinemia induced by L-methionine. Hyperhomocysteinemia was induced in wistar albino rats by methionine treatment (1 g/kg, p.o.) for 30 days. Folic acid (100 mg/kg, p.o.) given to rats as a standard drug treatment. Rats were fed with the aqueous extract of Nigella sativa (100 and 200 mg/kg, p.o.) for 30 days. The results of the present study after treatment with Nigella sativa aqueous extracts in two doses of 100 and 200 mg/kg body weight, showed lipid lowering, cardio- and neuro-protective potential of Nigella sativa. The results of test drug were compared with folic acid, a standard positive control. The present study results indicate that the aqueous extract of Nigella sativa seeds treatment protect the antioxidant defense against hyperhomocysteinemia, hyperlipidemia and oxidative stress in methionine-induced rat model.